Composite

Part:BBa_K552004:Design

Designed by: Jonathan Herrmann   Group: iGEM11_WashU   (2011-09-28)


CCD1 Gene-Codon Optimized for Yeast


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 771
    Illegal XbaI site found at 735
    Illegal XbaI site found at 753
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 771
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 771
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 771
    Illegal XbaI site found at 735
    Illegal XbaI site found at 753
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 771
    Illegal XbaI site found at 735
    Illegal XbaI site found at 753
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.


Source

The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm

References